Three controlled separation of substances microfluidic chip detection methods

High sensitivity for the detection of isolates microfluidic chip is of great significance. Currently, the detection method of microfluidic chips can be divided into three categories: optical detection, electrochemical detection, mass spectrometry detection. 

 

Ultraviolet Detection 

Ultraviolet absorption detector of a conventional optical detection method is a method corresponding detector has become mature, but because the chip channel is small, the sensitivity is not high, so that the method is not able to meet the very low concentrations and small sample analysis. 

 

Electrochemical Detection 

Electrochemical detection There are three basic modes: amperometric, conductivity and potentiometric method. The amp is the most common method of approach. The basic principle is: When measuring the compounds on the electrode surface by oxidation or reduction reactions, lose or get electrons to produce the electrode current is proportional to the analyte concentration, concentration of the solution can be obtained by measuring the changes in the current through the microchannel. The sensitivity of the electrochemical detection of the fluorescence can be detected with comparable results. 

 

MS detection method 

MS detection is based on the principle of different molecular mass to charge ratio to achieve the purpose of detection. Its biggest advantage is the ability to provide information about the molecular structure of space, it has unique in the study of the structure of biological macromolecules (such as proteins).